CT imaging is very useful in the development of bone scaffolding because it is necessary to see the scaffold in its entirety. Other methods such as a Scanning Electron Microscope (SEM) only offer surface imaging and require destructive sectioning of the sample. In this case, CT imaging allows us to see osteon like scaffolds on which cortical bone tissue can grow. Knowledge of artificial bone tissue scaffolding will be useful in developing bone grafting procedures for serious injuries.
The subject of the scan is a 3D scaffold made of Poly-L-lactide fibers on polyglycolide fibers. The fibers are spin together by electrospinning, then they are molded and sintered into the desired shape.
About the Scan
271 images were taken at 60 seconds per projection. The scan used a sweep angle of +- 135 degrees. Source voltage was at 30 kV with a current of 100 microamps. Each scan took approximately 5 hours. Samples were scanned in a .5 mL PCR tube. Scanning was performed by Kriti Sen Sharma.
Figure: Samples in their PCR tubes.
Links and Literature
Powerpoint presentation about the use of Micro CT in researching bone scaffolds: