This scan was performed on a mouse limb to reveal the capabilities of sub-microscale CT in scanning small animal parts. Dr. Steve Wang from Argonne National Labs sent the mouse limb to Virginia Tech to be scanned by Kriti Sen Sharma. The results were much better than those that would have been obtained with traditional microscale CT imaging.
The subject of the scan is a mouse limb. The way in which sub-micron resolution will represent the limb's bone structure and vasculature are of specific interest.
About the Scan
was performed with an Xradia MicroXCT 400 machine. The mouse limb was inserted
into a plastic tube to hold it in a fixed position during the scan. A preliminary scan at .5x was used to center the
tomography about the joint of the limb which was the point of interest. Next, a
scan from -90° to 90° was performed at 4X with a binning of 1 and a 120 second
exposure time. During the first scan the limb was able to vibrate in the tube, invalidating the reconstruction. Additionally, the limb dehydrated in the tube, causing it to contract. To correct these problems, the sample was put in a smaller tube packed with wet tissue paper to keep it hydrated. As a result, the limb was kept moist and stable enough during the scan to give a valid reconstruction.