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Steve Wang

Mouse Limb Scan - 12/21/2009  

Videos:

Organic Shading Video

A 360 degree view of the mouse joint using organic shading.



3D Section Video

A 3D planar section video showing the mouse joint in three different planes.


Axial Section Video

An axial section movie showing all of the slices comprising the joint.






    Scanner Settings             
 
Machine Xradia microXCT
Binning
1
Source Setting
40 kv, 100 µa
Source Distance
150 mm

Detector Distance

50 mm

Pixel Size
2.5626 µm

 FOV 10.4357 mm
Magnification 3.951X
 Exposure Time 120 s
 Image Size
 2018 X 2018
 Number of Projections
 361








Summary

This scan was performed on a mouse limb to reveal the capabilities of sub-microscale CT in scanning small animal parts. Dr. Steve Wang from Argonne National Labs sent the mouse limb to Virginia Tech to be scanned by Kriti Sen Sharma. The results were much better than those that would have been obtained with traditional microscale CT imaging. 

Subject

The subject of the scan is a mouse limb. The way in which sub-micron resolution will represent the limb's bone structure and vasculature are of specific interest. 

About the Scan

Scanning was performed with an Xradia MicroXCT 400 machine. The mouse limb was inserted into a plastic tube to hold it in a fixed position during the scan. A preliminary scan at .5x  was used to center the tomography about the joint of the limb which was the point of interest. Next, a scan from -90° to 90° was performed at 4X with a binning of 1 and a 120 second exposure time.  During the first scan the limb was able to vibrate in the tube, invalidating the reconstruction. Additionally, the limb dehydrated in the tube, causing it to contract. To correct these problems, the sample was put in a smaller tube packed with wet tissue paper to keep it hydrated. As a result, the limb was kept moist and stable enough during the scan to give a valid reconstruction.



Links and Literature

Argonne National Laboratory

www.anl.gov




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